The structure of CP-80,219, a new polyether antibiotic related to dianemycin.

describes the isolation, properties and structure of 1, which is related to dianemycin (2).X) The fermentation of S. hygroscopicus sp. ATCC 53626 and isolation of crude 1-Na as a hexane soluble oil (150g) was carried out as described elsewhere.2) This material was purified by HPLC on a Waters ^Bondapak C18 column using methanol water (90: 10), followed by HPLC on a silica gel column using methylene chloride ethanol (96 : 4). The eluates were examined by TLC on silica gel plates developed with chloroform 2-propanol (95 : 5), then sprayed with 3% vanillin dissolved in ethanol-phosphoric acid (2: 1). Upon heating to 80°C the polyether antibiotic appeared as a pink spot at Rf 0.19. Fractions containing 1-Na were combined and evaporated to dryness to afford 33 g of the desired product. The free acid form of 1 was prepared by vigorously shaking a chloroform solution of 1-Na with an equal 727